This paper concerns the study of prevalence of Type 1 latex protein allergy among workers in various latex glove factories in Malaysia. A total of 149 subjects (108 females and 41 males) with a mean age of 30.6 years were examined both by questionnaires and by skin prick test (SPT). In the absence of a standardised SPT latex allergen mixture, the clinical test was carried out using six glove extracts with extractable protein content varying from 0.02 mg/g to 0.75 mg/g of gloves (or 20 mg/g to 750 mg/g), as measured by the RRIM modified Lowry microassay. Only three subjects were found to show wheal size ranging from 2-4 mm when tested with glove extracts with extractable protein content of > 0.6 mg/g. Such reaction, was however not detected in all cases when protein levels were at 0.1 mg/g or lower. This prevalence as compared to those reported in the West is relatively low.