TY  - BOOK
AU  - Wajant H
AU  - Forster S
TI  - Purification and characterization of hydroxynitrile lyase from Hevea brasiliensis
PY  - 1996///
PB  - Plant science
KW  - Cyanogenesis
KW  - Enzyme purification
KW  - Hevea brasiliensis
KW  - Hydroxynitrile lyase
N2  - Hydroxynitrile lyase from Hevea brasiliensis (HbHNL) was purified to apparent homogeneity. Purified HbHNL consists of non-covalently linked monomers of 30kDa. HbHNL exhibits a typical Michaelis-Menten kinetics with a Km of 115 mM and the enzyme activity is strongly inhibited by diisopropyl fluorophosphate and diethyl pyrocarbonate indicating a serine and histidine in the active site. HbHNL is serologically related to the HNL from Manohot esculenta but not to other HNLs
ER  -