01338nam a2200133Ia 4500008004100000100002000041245014600061260004500207300001200252520087800264650002101142650002301163700001801186220216s9999    xx            000 0 und d  aCheong Kay Fong 0aNucleotide sequence of the chloroplast gene for the large sub-unit of ribulose-1,5-bisphosphate carboxylase/oxygenase from Hevea brasiliensis  bJournal of Natural Rubber Researchc1993  a146-153  aA shortgun genomic library of the chloroplast DNA (ctDNA) of Hevea brasiliensis (clone RRIM 600) was constructed with the plasmid cloning vector pUC19 in Escherichia coli. The gene, rbcL, for the large sub-unit (LS) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was isolated by first screening the library by colony hybridisation and then confirmation by Southern hybridization with a radiolabelled DNA probe that contains the internal sequence of rbcL from tobacco, Nicotiana tabacum. Sequence data indicated that the Hevea rbcL gene contains an open reading frame of 1425 nucleotides encoding a protein of 475 amino acid residues. Conserved sequences in the gene and LS protein were detected. Palindromic sequences which may form intrastrand stem-loop structures for the termination of transcription were encountered at the 3 non-coding region of the gene.  aChloroplast gene  aHevea brasiliensis  aKoh Chong Lek