TY  - BOOK
AU  - Guixiu Huang
AU  - Kunxin Wu
AU  - Shiqing Peng
AU  - Shoucai Chen
AU  - Xianghui Fu
TI  - mRNA differential display between healthy and TPD trees of Hevea brasiliensis
PB  - Proceedings of IRRDB Symposium 1999, Hainan, Peoples Republic of China. pp.337-344.
KW  - DDRT-PCR
KW  - mRNA
KW  - TPD hevea brasiliensis
N2  - Numerous studies have been carried out for the understanding of tapping panel dryness (TPD)disease, an extremely complex physiological syndrome affecting latex production in Hevea brasiliensis, which creating a serious economic handicap for rubber plantations. The cause of the disease, however, is still unknown although many different hypothesis have been proposed to explain it. In this study, the DDRT-PCR is used to search for differentially expressed genes between the healthy and TPD trees to understand the nature of the TPD phenomenon. Total RNAs of latexes and barks from both healthy and TPD trees, which belong to the clones RRIM600, 13-28, 1-20, PB5/51 X PR107, RRIM600 X PR107, were purified respectively and then subjected to DDRT-PCR. The result of displayed on sequencing gel showed that one marked common band appeared both latex and bark in all detected clones of healthy trees, but the band was very weak or not appeared in all tested TPD trees. The fragment proved to be positive by Northern Blot was cloned. Sequencing result showed that it is 725 bp long and no homologies are found. Further study may reveal the mechanism of the TPD disease occurring on Hevea brasiliensis when the expression of this gene is inhibited
ER  -