TY  - BOOK
AU  - Castonguay Yves
AU  - Bertrand Annick
AU  - Cloutier Jean
AU  - Dube Marie-Pier
AU  - Laberge Serge
AU  - Michaud Real
TI  - Molecular physiology and breeding at the crossroads of cold hardiness improvement
PY  - 2013///
PB  - Physiologia Plantarum
KW  - AFLP
KW  - Amplified fragment length polymorphism
KW  - BSA
KW  - Bulk segregant analysis
KW  - CBF
KW  - CNV
KW  - cold-regulated
KW  - Copy number variation
KW  - COR
KW  - c-repeat binding factor
KW  - c-repeat/dehydration responsive element
KW  - CRT/DRE
KW  - GBS
KW  - genotyping-by-sequencing
KW  - InDel
KW  - insertion and deletion
KW  - LD
KW  - linkage disequilibrium
KW  - marker-assisted selection
KW  - MAS
KW  - next-generation sequencing
KW  - NGS
KW  - QTL
KW  - quantitative trait loci
KW  - random amplified polymorphic DNA
KW  - RAPD
KW  - restriction fragment length polymorphism
KW  - RFLP
KW  - SCAR
KW  - sequence related amplified polymorphism
KW  - sequence-characterized amplified region
KW  - single-nucleotide polymorphism
KW  - SNP
KW  - SRAP
KW  - TF
KW  - tolerance to freezing
N2  - Alfalfa (Medicago sativa L) is a major forage legume grown extensively worldwide with important agronomic and environmental attributes. Insufficient cold hardiness is a major impediment to its reliable production in northern climates. Improvement of freezing tolerance using conventional breeding approaches is slowed by the quantitative nature of inheritance and strong interactions with the environment. The development of gene-based markers would facilitate the identification of genotypes with superior stress tolerance. Successive cycles of recurrent selection were applied using an indoor screening method to develop populations with significantly higher tolerance to freezing (TF). Bulk segregant analysis of heterogeneous TF populations identified DNA variations that are progressively enriched in frequency in response to selection. Polymorphisms resulting from intragenic variations within a dehydrin gene were identified and could potentially lead to the development of robust selection tools. Our results illustrate the benefits of feedback interactions between germplasm development programs and molecular physiology for a deeper understanding of the molecular and genetic bases of cold hardiness
ER  -