TY  - BOOK
AU  - Sunderasan E
AU  - Cardosa M J
AU  - Hamid Sharifah
AU  - Hamzah Samsidar
AU  - Ward M A
AU  - Yeang H Y
TI  - Latex B-Serum B-1,3-Glucanase (Hev b II) and a component of the microhelix (Hev b IV) are major latex allergens
PY  - 1995///
PB  - Journal of Natural Rubber Research
N2  - Two allergenic (IgE-binding) proteins have been isolated from the B-serum of natural rubber latex by dialysis-induced protein precipitation followed by gel filtration. One of the allergens, Hev b II, that is specific to the monoclonal antibody USM/RB4, commonly appears as a doublet of molecular weights 34 and 36 kDa under reducing conditions of SDS-polycacrylamide gel electrophoresis. In the absence of 2-mercaptoethanol, a 70 kDa band can also be seen in addition to the doublet. The allergen has a pI of circa 9.5 and is identified as B-1, 3-glucanase. The second allergen, Hev b IV, has a pI at of crica 4.5 and appears under under reducing conditions as a band of 50-57 kDa. In its unreduced form, a principal band of approximately 100 kDa is discernible. Immuno-gold labelling with the monoclonal antibody USM/RB3 shows that Hev B IV is a component of the microhelix protein complex of B-serum
ER  -