TY  - BOOK
AU  - Yeang H Y
AU  - Ghazaly H M
AU  - Sunderasan E
TI  - Latex allergy studies : Extraction of natural rubber latex proteins with reference to film thickness, latex D. R. C. and protein migration behaviour
PY  - 1995///
PB  - Journal of Natural Rubber Research
KW  - Film thickness
KW  - Latex allergy
KW  - Latex DRC
KW  - Natural Rubber Latex Proteins
KW  - Protein migration
N2  - Water-soluble proteins in wet natural latex films co-migrated with the evaporation stream to the surface when the film was dried at 100 C and moisture allowed to evaporate from one surface. When evaporation occurred from both surfaces of the latex film simultaneously, the evaporative pull from both directions appeared to annual each other resulting in little protein migration to either surface. Since unmigrated proteins are not readily extracted, the problem of allergenic proteins in latex films relateds essentially to the proteins that migrate to the surface. Wet-gel leaching of thin latex films (0.15 mm thick when dry) and thicker films (0.25- 0.35 mm) to remove soluble proteins was investigated by gel-leaching the films for 1-3 min with distilled water at room temperature after which they were completely dried at 100 C. Soluble proteins were reduced in thin films that were gel-leached. However, gel-leaching was counter-productive for the thicker films where extractable proteins increased. The amount of proteins extractable from latex films was also influenced by the d. r. c. of the latex used to prepare the films.Filims prepared from 40;d.r.c latex had significantly higher extractable proteins than filims of similar thickness that were prepared from 60;d.r.c. latex. Protein removal by wet-gel leaching was found not to be very effective because much of these proteins had not yet migrated to the surface when the film was heated briefly to attain the wet-gel state. When the wet-gel film was completely dried by prolonged heating after the leaching step, more proteins migrated to the surface. In the process of even brief 93 min or less) dry-film leaching, on the other hand, most of the soluble proteins had evidently migrated to the film surface at the time of leaching and their removal was hence much more effective
ER  -