TY  - BOOK
AU  - Kaplan Deborah A
AU  - Lytle
AU  - Myers Matthew R
AU  - Routson Licia B
TI  - Issues associated with using fluorescent microspheres to evaluate the barrier integrity of natural rubber latex films
PY  - 1999///
PB  - Journal of Rubber Research
KW  - Fluorescent polystyrene microspheres
KW  - Natural rubber Latex
KW  - NR latex condom
N2  - It has been reported that 100 nm - 110 nm fluorescent microspheres can pass through more natural rubber (NR) latex condoms than can the 27 nm virus &X174 as found by other investigators. Are there properties of fluorescent polystyrene microspheres that could be responsible for these disparate results? This study demonstrated that the microspheres adsorbed to a brand of condom made of NR latex as well as highly-adsorptive virus, PRDI ($X174 did not adsorb). The study also found that free fluorescent dye was not released when the microspheres were in close contact with the NR latex. These findings argue against microsphere properties were in close contact with the NR latex. These findings argue against microsphere properties being responsible for the disparate results. In addition, an increase in the fluorescence of buffer afer contact with NR latex condom was found, in the absence of microspheres. This increase may require extended contact. Thus, this study found an artifact that could be misconstrued as evidence of fluorescent microspheres passing through NR latex barriers
ER  -