TY  - BOOK
AU  - Sushamakumari S
AU  - Asokan M P
AU  - Jayasree R
AU  - Rekha K
AU  - Sobha S
TI  - Influence of growth regulators and sucrose on somatic embryogenesis and plant regeneration from immature inflorescence of Hevea brasiliensis
PY  - 2000///
PB  - Indian Journal of Natural Rubber Research
KW  - Growth regulators
KW  - Hevea brasiliensis
KW  - Plant regeneration
KW  - Somatic embryogenesis
N2  - A method for somatic embryogenesis and plant regeneration from immature inflorescence of Hevea brasiliensis, clone RRII 105 is described.  The influence of different growth regulators and sucrose at various stages of this pathway has also been investigated. Among the different growth regulators tested for callus induction, synergistic effect of the two auxins 2,4-D and NAA was observed. Optimum growth regulator combination for the callus induction was found to be 2,4-D/NAA/KIN (45/27/23 uM). Embryo induction was favoured by both BA and zeatin in the presence of low levels of NAA or 2,4-D. Significant increase in embryo induction frequency was observed in the presence of GA. A higher sucrose level was found to be essential for effective embryo induction as well as maturation. ABA did not show any positive effect on embryo maturation, eventhough lower levels of ABA in the maturation medium enhanced proliferation and further embryogenesis. Plant regeneration frequency was found to be higher on media fortified with BA and GA. Lower sucrose level was found to be beneficial for plant regeneration
ER  -