00927nam a2200133Ia 4500008004100000100002200041245007800063260004500141300001000186520054100196650001600737650002000753700002000773220216s9999    xx            000 0 und d  aShamsul Bahri A R 0aImmunocytochemical localisation of rubber membrane protein in Hevea latex  bJournal of Natural Rubber Researchc1996  a88-95  aImmunogold double labelling technique by electron microscopy was employed to localise the 14 kDa and 24 kDa protein obtained from rubber particle membrane in Hevea latex. These proteins were used to elicit antibodies in rabbits. The two antibodies showed selective labelling on the rubber particles: 14 kDa antibody bound more to the larger rubber particles (>_0.3 mm) whereas the 24 kDa antibody more to the smallerr rubber particles (<_ 0.2 mm). The use of osmium tetroxide to fix rubber particles did not distroy the antigenic sites.  aHevea latex  aRubber membrane  aHamzah Samsidar