TY  - BOOK
AU  - Wagner B
AU  - Breiteneder H
AU  - Buck D
AU  - Krebitz M
AU  - KyungHwan Han
AU  - Niggemann B
AU  - Scheiner
AU  - Yeet Yeang Hong
TI  - Cloning expression, and characterization of recombinant Hev b 3, a Hevea brasiliensis protein associated with latex allergy in patients with spina bifida
PY  - 1999///
PB  - Journal of Allergy and Clinical Immunology
KW  - Cloning
KW  - Hevea brasiliensis
KW  - Latex allergy
N1  - Source Year: 2000
N2  - A cDNA encoding Hev b 3 was amplified from RNA of fresh latex collected from Malaysian rubber trees and the 5- end sequence was obtained by specific amplification of cDNA ends. The recombinant Hev b 3 was produced in Escherichia coli as a 6xHis tagged protein. Immunoblotting and inhibition assays were performed to characterize the recombinant allergen. An Hev b 3 cDNA clone of 922 bp encoding a protein of 204 amino acid residues (22.3kDa) was obtained. It is concluded that recombinant Hev b 3 constitutes a suitable in vitro reagent for the diagnosis of latex allergy in patients with spina bifida. Nucleotide sequence data for the complete cDNA sequence of Hev b 3 have been deposited under EMBL accession number AJ223388
UR  - PBA
ER  -