TY  - BOOK
AU  - Sunderasan E
AU  - Lam K L
AU  - Ong M T
AU  - Rahman Norazreen Abd
AU  - Yang K L
TI  - Cell viability assay guided fractionation of natural rubber latex sera
PY  - 2013///
PB  - Journal of Rubber Research
KW  - anti-proliferative activity
KW  - Cell viability assay
KW  - Latex B-serum
KW  - Latex C-serum
N1  - Source Year: 2013
N2  - Natural rubber latex sera have been investigated for anti-proliferative property on cancer cell lines. The present report describes fractionaation of latex B- and C-serum using cell viability assay(anti-proliferative activity) as a guide to narrow down on the active constituents. Dialysis of B-and C-serum against distilled water caused some of its constituents to precipitate, then a brief centrifugation separated their fractions- dialysed B-serum percipitate (DCP) nad dialyesd C-serum  supernanatant (DCS). Boiling of B- and C-serum resulted in extensive precipitation; a brief centrifugation separated the boiled B-and C-serum precipitate from their supernantant. Cell viability assy performed on two human cancer-origin cell lines revealed a marked increase in anti-proliferative activity in the dialysed C-serum fractions and in DBP but diminished in DBS as well as in the boiled B-and C-serum fractions. Chromatographic separation of DBP, DCP and DCS followed by cell viability assy will shed light if sub-fractions with further improved anti-proliferative activity are attainable
ER  -