TY  - BOOK
AU  - Chow K S
AU  - Abdullah Latifah
AU  - Salimah Mohd Lim
AU  - Yusof Faridah
TI  - An Assessment of stimulation of Hevea brasiliensis rubber biosynthesis by eIF-5A protein-enriched bacterial lysates
PY  - 2006///
PB  - Journal of Rubber Research
KW  - cis-polyisoprene
KW  - eIF-5A
KW  - Glutathione-S-transferase
KW  - Isopentenyl diphosphate
KW  - Rubber biosynthesis
KW  - Stimulator protein
N2  - The effect of six unique isoforms of Hevea brasiliensis eukaryotic translation initiation factor 5A (eIF-5A), previously reported as a rubber biosynthesis stimulator protein (RBSP) was evaluated using biosynthesis assays containing zone 2 rubber particles and radiolabelled isopentenyl diphosphate ([14C]-IPP). Recombinant RBSP proteins were over-expressed as N-terminal glutathione-S-transferase (GST)-tagged proteins in a bacterial system. Crude extracts of six RBSP-GST isoforms were added to rubber biosynthesis assays in increasing volumes. Results showed that increases in the amount of each RBSP-GST isoform corresponded with increases in the amount of each RBSP-GST isoform corresponded with increases in the level of [14C]-IPP incorporation above that of the control which contained no recombinant protein. Statistical data analysis revealed that three RBSP isoforms increased [14C]-IPP incorporation significantly compared to the GST control where the RBSP isoform with the highest stimulatory effect produced a 45;increase in IPP incorporation compared to GST
ER  -