TY  - BOOK
AU  - Licy J
AU  - Bligh F J
AU  - Davey M R
AU  - Dickinson J
AU  - Power J B
AU  - Saraswathyamma C K
TI  - An Approach to identify disease resistance gene analogues in Hevea
PY  - 2000///
PB  - Indian Journal of Natural Rubber Research
KW  - Cloning
KW  - Hevea
KW  - Phytophtora leaf fall
KW  - POlymerase chain reaction
KW  - Resistance genes
N2  - A preliminary attempt was made to identify possible disease resistance gene analogues in rubber. A procedure was developed for the extraction of high quality DNA from rubber leaves, which can be used for the polymerase chain reaction (PCR) amplification of DNA. Eighteen primers, designed based on homologies between known resistance genes, were used in various combinations to amplify sequences from rubber cultivar FX 516, which is resistant to Phytophthora leaf fall disease and cultivar RRII 105, which is tolerant. The PCR products were cloned into plasmid vectors and the cloned inserts were sequenced. Although none of the clones obtained had high homology to resistance gene sequences, the putative protein encoded by one sequence had some homology to hem N gene
ER  -