In Cambodia, the common tapping system which is widely adopted by smallholders and agro-industry plantations is S/2 d3 for downward tapping and S/4 d3 for upward tapping. However, due to the decline of rubber price and increasing labour shortage, the application of low frequency tapping systems (LFT) may be a choice to solve the problems encountered. Hence, different LFT systems were tested in traditional rubber growing area of Cambodia. An experiment was established in a seven-year-old rubber field planted with PB 217 clone at the Cambodian Rubber Research Station located in Tbong Khmum Province. The experimental design was Randomised Complete Block Design with four treatments I.e. T0: S/2 d3 7d/7 ET 2.5% 10/y, T1: S/2 d4 7d/7 ET 2.5%, 14/y, T2: S/2 d5 7d/7 ET 3.3% 15/y and T3: S/2 d6 7d/7 ET 3.3% 20/y comprising four replications (16 elementary plots). After 8 years of tapping, LFT systems S/2 d6 with ethephon application (T3) provided the highest dry rubber yield per tree per tapping (g/t/t) but the lowest annual yield in gram per tree (g/t/y) and kilogram per hectare (kg/ha/y). Compared to d3, LFT systems (d4, d5 and d6) respectively showed 5, 9 and 14% loss in dry rubber yield per ha per year (ha/y), but with an increased labour productivity (g/t/t) by 24, 41 and 58%, respectively. Therefore, the increase in labour productivity (g/t/t) could compensate for the loss in land productivity (kg/ha). Girth increment was significantly different between treatments, in which LFT systems showed greater increments. Sucrose and reduced thiol contents of all treatments were significantly different depending on the tapping system, but inorganic phosphorus content was not significantly different. Tapping panel dryness was significantly different between treatments after 8 years of tapping.