The present study attempted preparation and evaluation of natural rubber (NR) samples from NR field latex with varying non-rubber compositions, derived through four different approaches. The approaches were designed in such as a way that stage wise removal of different non-rubber constituents from the rubber phase could be achieved. The study assessed the role of fragmented and non-fragmented protein components and other non-proteinaceous non-rubber components both under complete retention and partial retention within the rubber phase and its effect on various technological properties of the rubber composites. Nitrogen content of the rubber samples subjected to the four different approaches varied from 0.57 to 0.90 per cent. The preparation of NR-HAF carbon black composites with the above rubber samples and evaluation of its cure behavior and technological properties were carried out. The thermo-oxidative ageing behavior of the corresponding NR composites was also evaluated. This study showed that the content of non-rubber constituents in NR has a significant role in improving the processability, determining the cure rate and cure state of the rubber compounds and influencing technological properties of rubber vulcanizates. Most remarkably, ageing resistance of the vulcanizates improved significantly with increasing content of proteins, both in its fragmented and non-fragmented states in NR.