To meet its rising demand natural rubber cultivation is being extended to the North Eastern (NE) regions of India where low temperature is a major growth limiting factor. Among the non-traditional areas, there is greater focus on expanding rubber cultivation in the NE region. In the present study, low temperature responses were evaluated in five clones of Hevea brasiliensis. Young plants (six to eight-month-old) were grown under ambient tropical conditions successively exposed to low temperature for 14 days by a gradual reduction of temperature from 30o C/22o C to 15o C/7o C and allowing a stress recovery period of five days at 30o C/22o C under controlled condition. Cold stress treatment significantly affected growth in the experimental clones. However, three clones under stress condition viz. SCATC 88/13, RRII 208 and RRIM 600 showed better leaf and stem dry weight (DW). Leaf mass fraction (LMF), stem mass fraction (SMF) and leaf thiol content levels were higher in SCATC 88/13, RRIM 600 and RRII 208. When exposed to low temperature there was a reduction in the content of photosynthetic pigments, maximum photochemical efficiency (Fv/ Fm) and effective quantum yield of PSII (ΦPSII) in all the genotypes; however, reduction was the least in SCATC 88/13 and RRIM 600. During stress recovery the rate of PSII repairing was better in three clones viz. SCATC 88/13, RRII 208 and RRIM 600. Although most of the clones showed sensitivity to low temperature stress, there was variations in cold response and recovery capabilities of the clones in terms of growth and photosynthetic parameters. Overall, clone SCATC 88/13 followed by RRIM 600 and RRII 208 had better tolerance to cold stress.