Room temperature prevulcanized natural rubber latex prepared by a combination of accelerator systems was investigated with zinc diethyl dithiocarbamate/zinc dibutyl dithiocarbamate/sodium dibutyl dithio carbamate/zinc isopropyl xanthate/zinc 2-mercaptobenzothiazole (ZDEC/ZDBC/SDBC/ZIX/ZMBT). The colloidal stability and mechanical properties of the room temperature prevulcanized latex compound was investigated. The mechanical properties of prevulcanized latex compound was compared with that of a ZDEC system using heating method 60 degree C for 4h. The first combination of room temperature system using ZDEC/ZDBC/ZMBT (RT1), second ZDEC/ZIX/ZMBT (RT2) and third ZDEC/SDBC/ZMBT (RT3). The test result showed that RT1, RT2, and RT3 can bring about prevulcanization at room temperature. The latex film prepared from the room temperature prevulcanized latex compound gives better clarity than conventional prevulcanized samples.