Hevea brasiliensis, the natural rubber tree is mainly cultivated in traditional regions like Kerala and Kanyakumari District of Tamil Nadu and to a lesser extent in non-traditional regions like Karnataka, Maharashtra, Madhya Pradesh, Orissa and North-Eastern states of India. These regions experience either drought coupled with high temperature in summer or cold in winter seasons. Abiotic stress tolerant clones newly developed by RRII are in the pipeline for evaluation of their field performance in various agro-climatic zones. In the present study, 45 pipeline clones grown in polybags at Regional Research Stations situated at North Konkan Region (Maharashtra) and Agarthala (Tripura) were allowed to experience drought stress during summer and low temperature stress during winter respectively. The effect of stress on these clones was assessed by measuring various physiological parameters. In terms of CO2 assimilation, the pipeline clones P 20, P 73 and P 57 performed better than the tolerant check clones RRIM 600, RRII 430 and RRII 208 under drought conditions. Percentage of drought influenced inhibition in CO2 assimilation was at minimal in these clones. In terms of transpiration and photosynthetic efficiency, the clones P 20 and P 73 performed better while P 20, P 21 and P 73 displayed least membrane injury and stayed green under drought stress. In terms of overall performance, P 20 and P 73 were found superior in drought prone region. Similarly under cold stress, performance of P 078, P 083, P 01 and P 101 was found on par with the check clones RRII 429 and RRII 208 in terms of CO2 assimilation. In terms of photosynthetic efficiency, P 01, P 71, P 83 and P 76 were found superior under cold stress. In total, the clone P 01 was found superior under cold stress in terms of parameters investigated. This multi-locational study could identify potential drought and cold tolerant pipeline clones. This data only when integrated with mature yield data of these clones would indicate the practical utility of identifying clones suitable for cultivation in stress prone regions.