Lutoidic 35.5-kDa protein as a possible molecular marker of latex productivity in Hevea brasiliensis
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TextPublication details: IRRDB symposium on physiological and molecular aspects of the breeding of Hevea brasiliensis 6-7 November 1995 1995Description: 31-41Subject(s): Summary: Classical methods appear to be limited in discrimination of production variations and hence a molecular approach was chosen for our two-stage study with the main goal of identifying production markers. The first stage consisted of searching for proteins related to the two main production factors in Hevea, namely tapping frequency and stimulation intensity. The second stage consisted of following these potential marker-proteins in order to establish a relationship, on the one hand, between these and clonal typology (metabolism), and, on the other hand, between inter- and intraclonal production variations and the same markers. Total soluble proteins of latex compartments were analysed using one-dimensional (1-D) and two-dimensional (2-D) polyacrylamid gel electrophoresis (PAGE) in denaturing conditions.
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RRII Library Physiology | Journals |
Classical methods appear to be limited in discrimination of production variations and hence a molecular approach was chosen for our two-stage study with the main goal of identifying production markers. The first stage consisted of searching for proteins related to the two main production factors in Hevea, namely tapping frequency and stimulation intensity. The second stage consisted of following these potential marker-proteins in order to establish a relationship, on the one hand, between these and clonal typology (metabolism), and, on the other hand, between inter- and intraclonal production variations and the same markers. Total soluble proteins of latex compartments were analysed using one-dimensional (1-D) and two-dimensional (2-D) polyacrylamid gel electrophoresis (PAGE) in denaturing conditions.
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