Latex flow after tapping and regeneration of cellular materials before the next tapping are the two main factors that limit rubber production in Hevea brasiliensis. Latex production is controlled by different mechanisms such as sucrose loading to laticifers, availability of biochemical energy, membrane transport, pH regulation and the capacity of laticifers to regenerate the cellular materials lost during tapping. The synthesis and turnover of proteins is very important in the overall metabolic rate. Latex regeneration mechanism of twelve clones of Hevea brasiliensis with different yield potentials were studied in relation to carbohydrate metabolism, lutoid membrane transport and protein synthesis. Rubber yield, sucrose, ATP, invertase in latex, H+ ATPase, H+ pyrophosphatase activity of lutoid membrane and protein biosynthetic capacity of each clone was measured during peak yielding season. High activity of membrane enzymes and availability of biochemical energy was observed in clones RRII 105, RRIM 600, PB 260, RRII 422 and RRII 430 than other clones. Protein biosynthetic capacity was highest in clone PB 260 and RRIM 600. The very low sugar content and increased invertase activity of clone PB 260 indicate intense metabolic activity and high protein biosynthetic capacity of this clone indicated efficient regeneration of cellular materials lost during tapping. Clone PB 217 is characterized by very high sucrose and low ATP and ATPase indicating slow metabolism and this clone may well respond to stimulation. Clone GT-1 showed a high ATP, invertase and protein synthesis compared to low yielding clones. Low yielding clones (RRII 33 and RRII 38) always showed a high sucrose but very low activities of membrane enzymes, ATP and protein synthesis. Among RRII 400 series clones, latex regeneration capacity was high in RRII 422 and RRII 430. The significance of carbohydrate metabolism, membrane transport and protein synthesis is discussed in relation to general metabolic activity of these clones and to propose suitable tapping systems and stimulation methods to each clone.