A preliminary investigation to optimize the conditions for successful in vitro fertilization and subsequent embryo development in Hevea brasiliensis has been conducted. The pollination method, stage of the flower and cluster medium for effective fertilization have been optimized. Ovular pollination has been identified as the suitable method of in vitro pollination in Hevea brasiliensis. The flowers one day before anthesis were identified as the optimum stage for in vitro pollination. The fertilized ovules could be grown up to three months and embryos were grown up to the heart shaped stage. This is the first report in Hevea brasiliensis. MS medium supplemented with complex organic nutrients viz coconut water, case in hydrolysate, malt extract, banana powder and extract prepared from Hevea endosperm promoted ovule/embryo development. The growth regulators GA3 (1.0mg/1) and KIN (2.0mg/1) also improved the ovule/embryo development. Anatomical studies of the developing ovules revealed the abundance of starch and lipids in the nucellar area, 30 days after pollination. A well developed seed coat was observed at 90 DAP. The morphology of 90 day oild developing seed after in vitro fertilization resembled a mature seed in vivo.