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Electrophoresis application to rubber clone identification

By: Contributor(s): Material type: TextTextPublication details: IRRDB symposium on physiological and molecular aspects of the breeding of Hevea brasiliensis 6-7 November 1995 1995Description: 69-73Subject(s): Summary: Large-scale multiplication of rubber planting material that is not true-to-type can seriously jeopardize the economic profitability of plantations. Isozyme electrophoresis has been developed to characterize clone genotypes at an early stage in their growth. This technique has proved particularly effective with a 98;separation capability for the main clones cultivated. As the equipment required is simple and operating costs are relatively low, rubber clone identification using this technique has become routine. A mobile electrophoresis kit or portable laboratory has been devised for direct conformity checks in budwood gardens on plantations. It has been successfully used in C
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Large-scale multiplication of rubber planting material that is not true-to-type can seriously jeopardize the economic profitability of plantations. Isozyme electrophoresis has been developed to characterize clone genotypes at an early stage in their growth. This technique has proved particularly effective with a 98;separation capability for the main clones cultivated. As the equipment required is simple and operating costs are relatively low, rubber clone identification using this technique has become routine. A mobile electrophoresis kit or portable laboratory has been devised for direct conformity checks in budwood gardens on plantations. It has been successfully used in C te dIvoire, Indonesia, French Guiana, Guadeloupe, Guatemala, Brazil and Cambodia on many individuals. Expanded use is proposed through assessment missions or technology transfer.

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