Chlorination at room temperature can accelerate the reduction of nitrosamine and nitrostable contents of natural rubber gloves. The extent of the reduction for double-surface chlorinated gloves was founf to tbe higher than that for single-surface chlorinated gloves. When compared to leaching of the rubber glove at room temperature, chlorination can remove much more nitrosamine and nitrostable substances from the glove. A study of the model reaction between nitrosamine and chlorine indicated that the positive effects of chlorination on the reduction of nitrosamine and nitrostable concentrations were possibly due to the formation of a more soluble salt in the presence of chloride in the acidic medium. A survey of commercial chlorinated and powdered gloves produced by the same manufacturer indicated that the nitrosamine content of chlorinated gloves was higher than that of powdered gloves. The discrepancy could be due to different formulations and processing conditions used in producing the two different types of gloves.