The development of molecular markers for Hevea breeding (Record no. 70573)

MARC details
000 -LEADER
fixed length control field 01944nam a2200241Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Low F C
245 #4 - TITLE STATEMENT
Title The development of molecular markers for Hevea breeding
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher IRRDB symposium on physiological and molecular aspects of the breeding of Hevea brasiliensis 6-7 November 1995
Year of publication 1995
300 ## - PHYSICAL DESCRIPTION
Number of Pages 18-Aug
520 ## - SUMMARY, ETC.
Summary, etc Four DNA molecular marker techiques are described. These are the classical hybridisation-based molecular marker technique called restriction fragment length polymorphisms (RFLPs) and three in vitro amplification-based techniques,namely random amplified polymorphic DNA (RAPD), microsatellites or simple sequence repeats (SSRs) and DNA amplification fingerprinting (DAF). These molecular markers were able to elicit interspecific polymorphisms. They were also able to assist in sveral identification studies. Cultivars wich share two common parents (siblings), such as PR 255 and PR 261 ; RRIM 901 and RRIM 905 ; and RRIM 937 and RRIM 938, have been distinguished by their DNA polymorphisms. Similarly, molecular markers have assisted in the identification of rouges in a sample of monoclonal PB 5/51 seedilings. The genetic diversity of genotypes from the 1981 IRRDB Hevea germplasm collectio from Brazil was also demonstrated by their RFLPs. DNA fingerprinting techniques such as SSR and DAF have elicited somaclonal variants among a population of GL 1 plants derived from anther and ovule culture. A genetic linkage map comprising of RFLPs and other markers is being attempted. The strength and limitations of these marker techniques are discussed and compared.
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Topical Term Breeding
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Topical Term DNA fingerprinting
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Topical Term Hevea
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Topical Term molecular markers
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Topical Term RAPD
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Topical Term RFLP
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Topical Term Simple sequence repeats (SSRs)
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Atan Safiah
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Personal name Jaafar Hafsah
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Personal name Tan H
942 ## - ADDED ENTRY ELEMENTS (KOHA)
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