Purification and characterization of phosphoribosylpyrophosphate synthetase from rubber tree latex (Record no. 66109)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 01748nam a2200181Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Gallois Richard |
| 245 #0 - TITLE STATEMENT | |
| Title | Purification and characterization of phosphoribosylpyrophosphate synthetase from rubber tree latex |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Plant Physiology |
| Year of publication | 1997 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 847-852 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Phosphoribosylpyrophosphate synthetase (PRS; EC 2.7.6.1) from Hevea brasiliensis Muell. Arg. latex was located in the cytosol. After purification, its apparent molecular weight under nondenaturing conditions was estimated at 200,000+_ 10,000; a single band at 57,000 +_ 3,000 was detected after sodium dodecyl sulfatepolyacrylamide gel electrophoresis. The enzyme seemed to be a homotetramer. Its affinity constants were estimated at 200 +_ 30 mM for adenosine triphosphate and 40 +_ 2 mM for ribose-5-phosphate. The purified enzyme proved to be functional in a paraphysiological medium (cytosol deproteinized by ultrafiltration). Optimum pH was 7.5 in buffer and 6.5 in a paraphysiological medium. No PRS activity was detected in the absence of the Mg2+ ion. Of the numerous compounds tested, only Mn2+, phosphoribosylpyrophosphate, and inorganic phosphate affected the enzymatic reaction. Mn2+ (inhibitor constant = 20 mM) and phosphoribosylpyrophosphate (inhibitor constant = 30mM) were inhibitors. PRS responded allosterically (Hills coefficient = 2.3) to ribulose-5-phosphate in the presence of a physiological concentration of inorganic phosphate (10mM). These results are set in the physiological context of laticifers. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Phosphoribosylpyrophosphate synthetase |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Rubber tree latex |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Clement A |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jacob J-L |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prevot J-C |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Physiology | 17/12/2021 | Volume 115, Issue 2 | Journals |