Purification and characterization of hydroxynitrile lyase from Hevea brasiliensis (Record no. 66108)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 00962nam a2200181Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Wajant H |
| 245 #0 - TITLE STATEMENT | |
| Title | Purification and characterization of hydroxynitrile lyase from Hevea brasiliensis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Plant science |
| Year of publication | 1996 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 25-31 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Hydroxynitrile lyase from Hevea brasiliensis (HbHNL) was purified to apparent homogeneity. Purified HbHNL consists of non-covalently linked monomers of 30kDa. HbHNL exhibits a typical Michaelis-Menten kinetics with a Km of 115 mM and the enzyme activity is strongly inhibited by diisopropyl fluorophosphate and diethyl pyrocarbonate indicating a serine and histidine in the active site. HbHNL is serologically related to the HNL from Manohot esculenta but not to other HNLs. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Cyanogenesis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Enzyme purification |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hydroxynitrile lyase |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Forster S |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Physiology | 17/12/2021 | Volume 115, Issue 1 | Journals |