Proteins of dialysed C-serum supernatant sub-fractions elicit anti-proliferative activity on human cancer-origin cells (Record no. 66064)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 01529nam a2200193Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Sunderasan E |
| 245 #0 - TITLE STATEMENT | |
| Title | Proteins of dialysed C-serum supernatant sub-fractions elicit anti-proliferative activity on human cancer-origin cells |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Journal of Rubber Research |
| Year of publication | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 49-59 |
| 500 ## - GENERAL NOTE | |
| General note | Source Year: 2015 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Dialysed latex C-serum fractions have been investigated for anti-proliferative activity against human cancer origin cell lines. The present report describes further fractionation of dialysed C-serum supernatant (DCS) and analysis of the sub-fractions that elicit anti-proliferative activity on HepG2 and MDA-MB231, the test cancer origin cell lines. DCS was fractionated through a Sephacryl S-200 column, and the eluents were pooled into four sequential subfractions, named DCS-F1, -F2, -F3 and -F4. Cell viability assay revealed that anti-proliferative activity was confined to DCS-F2 and -F3. Furthermore an improved anti-proliferative activity was discerned in the recombined active DCS sub-fractions. DCS-F2 and -F3 were subjected to two dimensional polyacrylamide gel electrophoresis, subsequent MALDI-TOF/TOF analysis and database searches revealed the presence of a number of proteins that have been shown to be implicated in anti-cancer activity. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Cell viability assay |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Electrophoresis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Gel-filtration |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Latex C-serum |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Proteins |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Rubber technology | 22/06/2015 | Volume 18, Issue 1 | Journals |