Nucleotide sequence of the chloroplast gene for the large sub-unit of ribulose-1,5-bisphosphate carboxylase/oxygenase from Hevea brasiliensis (Record no. 64217)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01386nam a2200157Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Cheong Kay Fong |
| 245 #0 - TITLE STATEMENT | |
| Title | Nucleotide sequence of the chloroplast gene for the large sub-unit of ribulose-1,5-bisphosphate carboxylase/oxygenase from Hevea brasiliensis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Journal of Natural Rubber Research |
| Year of publication | 1993 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 146-153 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | A shortgun genomic library of the chloroplast DNA (ctDNA) of Hevea brasiliensis (clone RRIM 600) was constructed with the plasmid cloning vector pUC19 in Escherichia coli. The gene, rbcL, for the large sub-unit (LS) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was isolated by first screening the library by colony hybridisation and then confirmation by Southern hybridization with a radiolabelled DNA probe that contains the internal sequence of rbcL from tobacco, Nicotiana tabacum. Sequence data indicated that the Hevea rbcL gene contains an open reading frame of 1425 nucleotides encoding a protein of 475 amino acid residues. Conserved sequences in the gene and LS protein were detected. Palindromic sequences which may form intrastrand stem-loop structures for the termination of transcription were encountered at the 3 non-coding region of the gene. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Chloroplast gene |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Koh Chong Lek |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 17/12/2021 | Volume 8, Issue 2 | Journals |