Molecular, physiological and pathological characterization of Corynespora leaf spot fungi from rubber plantations in Sri Lanka (Record no. 63230)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01877nam a2200193Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Silva W P K |
| 245 #0 - TITLE STATEMENT | |
| Title | Molecular, physiological and pathological characterization of Corynespora leaf spot fungi from rubber plantations in Sri Lanka |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Plant Pathology |
| Year of publication | 1998 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 267-277 |
| 500 ## - GENERAL NOTE | |
| General note | Source Year: 1998 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Genetic variability in 32 isolates of Corynespora cassiicola collected from diverse hosts (27 from Hevea brasiliensis) and locations in Sri Lanka and Australia was assessed using restriction fragment length polymorphism (RFLP) analysis of the internal transcribed spacer (ITS) region of ribosomal DNA and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis of total fungal DNA. Amplified ITS fragments from all 32 C. cassiicola isolates exhibited an identical size, and restriction analysis with 7 different restriction endonucleases revealed identity in all of the detected DNA fragments. This finding of high genetic relatedness was further supported by the cloning and DNA sequencing of the ITS2 region from one Sri Lankan and one Australian isolate. However, RAPD-PCR profiles generated by 15 oligonucleotide decamer primers revealed significant polymorphism between groups of organisms. Genetic relationships among the isolates were determined by cluster analysis of the RAPD-PCR data and 7 different RAPD groups were identified. Isolates showed strong correlations between the assigned RAPD group and the location and host plant genotype from which the isolate was collected. Correlations were also observed between the RAPD group, growth of the isolate and pathogenicity on different plant hosts. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Corynespora |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Rubber plantation |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Deverall B J |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lyon B R |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | RPP |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Pathology | 01/01/2021 | Volume 47, Issue 3 | Journals |