Micropropagation of rubber trees (Hevea brasiliensis Muell. Arg.) (Record no. 62928)

MARC details
000 -LEADER
fixed length control field 02025nam a2200193Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Mendanha Asseara Batista Lettao
245 #0 - TITLE STATEMENT
Title Micropropagation of rubber trees (Hevea brasiliensis Muell. Arg.)
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher Genetics and Molecular Biology
Year of publication 1998
300 ## - PHYSICAL DESCRIPTION
Number of Pages 395-398
500 ## - GENERAL NOTE
General note Source Year: 1999
520 ## - SUMMARY, ETC.
Summary, etc Tissue cultures were established from newly expanded leaves and axillary buds of rubber trees (Hevea brasiliensis Muell. Arg.) . Calli formed from these explants, but no regeneration occurred. Shoots were obtained from axillary buds cultured on Murashige and Skoogs (MS) medium supplemented with 1.0 mg/l kinetin, 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 20 g/l sucrose and 4 g/l Difco agar. Formation of a root similar to a tap root was induced on MS medium supplemented with 5.0 mg/l naphthaleneacetic acid (NAA), 3.0 mg/l inodlylbutyric acid (IBA), 50 g/l sucrose and 4 g/l Difco agar. Several types of explants were used in attempts to recover complete rubber tree plants with well-developed tap roots. Leaf explants and axillary buds formed calli on MS basic medium with different combinations of kinetin, benzylaminopurine (BAP), 2,4-D, IBA, NAA and indolylacetic acid (IAA). The antibiotic tetracycline was also used to control possible bacterial infections. However, no antibiotic effect was noted. Calli formation was abundant, but no regeneration was observed when the calli from different media was transferred to MS medium without growth hormones. On this basic medium, callus cultures became necrotic and died. Shoots developed from axillary buds, rooted vigorously when cultured on MS medium with NAA, IAA, and IBA. Based on these results, further studies with commercially important clones should lead to a feasible micropropagation technique.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Micropropagation
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Topical Term Rubber tree
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Personal name Freire
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Torres Roberto Augusto de Almeida
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier PGRA
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Journals
700 ## - ADDED ENTRY--PERSONAL NAME
-- Adelson de Barros
Holdings
Withdrawn status Lost status Damaged status Not for loan Home library Current library Shelving location Date acquired Serial Enumeration / chronology Koha item type
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