Latex B-Serum B-1,3-Glucanase (Hev b II) and a component of the microhelix (Hev b IV) are major latex allergens (Record no. 61993)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01424nam a2200181Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Sunderasan E |
| 245 #0 - TITLE STATEMENT | |
| Title | Latex B-Serum B-1,3-Glucanase (Hev b II) and a component of the microhelix (Hev b IV) are major latex allergens |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Journal of Natural Rubber Research |
| Year of publication | 1995 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 82-99 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Two allergenic (IgE-binding) proteins have been isolated from the B-serum of natural rubber latex by dialysis-induced protein precipitation followed by gel filtration. One of the allergens, Hev b II, that is specific to the monoclonal antibody USM/RB4, commonly appears as a doublet of molecular weights 34 and 36 kDa under reducing conditions of SDS-polycacrylamide gel electrophoresis. In the absence of 2-mercaptoethanol, a 70 kDa band can also be seen in addition to the doublet. The allergen has a pI of circa 9.5 and is identified as B-1, 3-glucanase. The second allergen, Hev b IV, has a pI at of crica 4.5 and appears under under reducing conditions as a band of 50-57 kDa. In its unreduced form, a principal band of approximately 100 kDa is discernible. Immuno-gold labelling with the monoclonal antibody USM/RB3 shows that Hev B IV is a component of the microhelix protein complex of B-serum. |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cardosa M J |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hamid Sharifah |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hamzah Samsidar |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ward M A |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yeang H Y |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Rubber chemistry | 17/12/2021 | Volume 10, Issue 2 | Journals |