Influence of growth regulators and sucrose on somatic embryogenesis and plant regeneration from immature inflorescence of Hevea brasiliensis (Record no. 61238)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 01771nam a2200217Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Sushamakumari S |
| 245 #0 - TITLE STATEMENT | |
| Title | Influence of growth regulators and sucrose on somatic embryogenesis and plant regeneration from immature inflorescence of Hevea brasiliensis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Indian Journal of Natural Rubber Research |
| Year of publication | 2000 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 19-29 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | A method for somatic embryogenesis and plant regeneration from immature inflorescence of Hevea brasiliensis, clone RRII 105 is described. The influence of different growth regulators and sucrose at various stages of this pathway has also been investigated. Among the different growth regulators tested for callus induction, synergistic effect of the two auxins 2,4-D and NAA was observed. Optimum growth regulator combination for the callus induction was found to be 2,4-D/NAA/KIN (45/27/23 uM). Embryo induction was favoured by both BA and zeatin in the presence of low levels of NAA or 2,4-D. Significant increase in embryo induction frequency was observed in the presence of GA. A higher sucrose level was found to be essential for effective embryo induction as well as maturation. ABA did not show any positive effect on embryo maturation, eventhough lower levels of ABA in the maturation medium enhanced proliferation and further embryogenesis. Plant regeneration frequency was found to be higher on media fortified with BA and GA. Lower sucrose level was found to be beneficial for plant regeneration. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Growth regulators |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Plant regeneration |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Somatic embryogenesis |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Asokan M P |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jayasree R |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rekha K |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sobha S |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 17/12/2021 | Volume 13, Issue 02-Jan | Journals |