In vitro characterization and in vivo detection of Rigidoporus lignosus, the casual agent of white root disease in Hevea brasiliensis, by ELISA techniques (Record no. 60940)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02114nam a2200265Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Louanchi M |
| 245 #0 - TITLE STATEMENT | |
| Title | In vitro characterization and in vivo detection of Rigidoporus lignosus, the casual agent of white root disease in Hevea brasiliensis, by ELISA techniques |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | European Journal of Plant Pathology |
| Year of publication | 1996 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 33-44 |
| 500 ## - GENERAL NOTE | |
| General note | Source Year: 1996 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | A method for early detection of R. lignosus was developed. Two polyclonal sera were produced against soluble mycelial proteins of 2R. lignosus isolates, 1 from Africa (FCI2), the other from Asia (FID2). The specificity of the antisera was tested using isoelectric focusing (IEF)/Western-bolt and DAS-ELISA. The 2 sera recognized all 20 R. lignosus isolates from various geographical origins. The banding patterns obtained by Western-bolt enabled a distinction to be made between isolates from Africa and Asia. In DAS-ELISA and Western-bolt analyses, strong cross reactions were observed with R. ulmarius. Only slight reactions were observed in Western-blot analysis to R. lineatus and Phellinus noxius, both causative agents of root rot in Hevea. Cross reactions were not observed under the DAS-ELISA analysis conditions. No cross reactions were obtained with 9 other Polyporaceae or Hevea root pathogen species. The sensitivity threshold of the DAS-ELISA method was 5 ng/ml of R. lignosus protein. An initial approach to using the DAS-ELISA test for the detection of R. lignosus in infected plants was carried out on artificially inoculated root samples. The DAS-ELISA protocol enabled detection of R. lignosus in the root system of diseasesd plants. No cross reaction was observed with healthy plant extracts. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | DAS-ELISA techniques |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | In vitro characterization |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | In vivo detection |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Rigidoporuslignosus |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | White root disease |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Balesdent M H |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Despreaux D |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Michels T |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Robin P |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | RPP |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Pathology | 17/12/2021 | Volume 102, Issue 1 | Journals |