In vitro antifungal activity of recombinant chitinase protein against Corynespora cassiicola infecting Hevea brasiliensis (Record no. 60937)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02014nam a2200229Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Philip S |
| 245 #0 - TITLE STATEMENT | |
| Title | In vitro antifungal activity of recombinant chitinase protein against Corynespora cassiicola infecting Hevea brasiliensis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Rubber Science |
| Year of publication | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 130-137 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Corynespora leaf disease caused by Corynespora cassiicola has emerged as a major disease of rubber in South East Asia. During plant-pathogen interactions, various novel proteins called pathogenesis related (PR) proteins which play a major role in plant disease resistance mechanism are induced. The chitinase (PR3) is one of the most widely studied groups of PR proteins in plants. C.cassiicola induced chitinase from Hevea brasiliensis was characterised in the present study. The cDNA was developed from the RNA of C. cassiicola infected leaves of the clone GT 1. A 978 bp chitinase gene was obtained from H. brasiliensis and over expressed in the pET 32a+ expression system. The in vitro studies of purified recombinant Hevea chitinase showed antifungal activity against C. cassiicola. The chitinase gene expression in H. brasiliensis during C. cassiicola infection was quantified through qPCR and increased expression of chitinase transcripts was observed. In clone GT 1 chitinase gene was induced up to 24th hour after C.cassiicola infection and eventually it came down in later hours, where as in clone RRII 105 chitinase level had been lesser in induced plants than control. The polyclonal antibody was raised with the recombinant chitinase and the induced clone GT 1 showed a prominant band in western blot, while a minor band was observed in RRII 105 in induced condition. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Chitinase |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Corynespora cassiicola |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Recombinant protein |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abraham A |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Joseph A |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prem E |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sathik M B M |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Thomas K U |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Pathology | 17/12/2021 | Volume 28, Issue 2 | Journals |