Identification of Colletotrichum acutatum from rubber using random amplified polymorphic DNAs and ribosomal DNA polymorphisms (Record no. 60621)

MARC details
000 -LEADER
fixed length control field 01993nam a2200217Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Saha Thakurdas
245 #0 - TITLE STATEMENT
Title Identification of Colletotrichum acutatum from rubber using random amplified polymorphic DNAs and ribosomal DNA polymorphisms
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher Mycological Research
Year of publication 2002
300 ## - PHYSICAL DESCRIPTION
Number of Pages 215-221
520 ## - SUMMARY, ETC.
Summary, etc The fungal pathogen responsible for Colletotrichum leaf disease of Hevea, develops three different disease symptoms: raised spots, anthracnose and papery lesions. These diseases have been attributed to Colletotrichum gloeosporioides (teleomorph Glomerella cingulata) and the fungi involved are morphologically indistinguishable. Twenty-five Colletotrichum isolates, which originated from three different disease symptoms, were characterized initially using RAPD markers. Two major RAPD profiles were detected which were related to the type of disease symptom developed. Colletotrichum isolates causing raised spot symptom were easily distinguished from isolates originating from either anthracnose or papery lesions. Restriction analysis of the PCR amplified 5.8S ribosomal DNA (rDNA), including both the flanking internal transcribed spacers (ITS) of representative isolates from the three different disease symptoms, also revealed two distinct RFLP patterns reflecting the same groupings as detected through RAPDs. Both molecular approaches suggested that there were two species of Colletotrichum associated with Hevea inciting the development of three different symptoms: Colletotrichum acutatum causing raised spot symptom, and C. gloeosporioides causing both anthracnose and papery lesions. This is the first record of Colletotrichum acutatum on Hevea in India.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Colletotrichum acutatum
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term DNA polymorphism
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Arun Kumar
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Bindu Roy
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Jacob Kuruvilla
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Minimol Ravindran
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Nazeer M A
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier Reprint
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Journals
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