Hydroxynitrile lyase from Hevea brasiliensis: Molecular characterization and mechanism of enzyme catalysis (Record no. 60558)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01368nam a2200205Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Hasslacher M |
| 245 #0 - TITLE STATEMENT | |
| Title | Hydroxynitrile lyase from Hevea brasiliensis: Molecular characterization and mechanism of enzyme catalysis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Proteins |
| Year of publication | 1997 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 438-449 |
| 500 ## - GENERAL NOTE | |
| General note | Source Year: 1998 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | (S)-Hydroxynitrile lyase (Hnl) from the rubber tree Hevea brasiliensis is a 29 kDa single chain protein that catalyses the breakdown or formation of a C(SINGLE BOND)C bond by reversible addition of hydrocyanic acid to aldehydes or ketones. The primary sequence of Hnl has no significant homology to known proteins. Detailed homology investigations employing PROFILESEARCH and secondary structure prediction algorithms suggest that Hnl is a member of the hydrolase fold protein family and contains a catalytic triad as functional residues was tested and confirmed by site-directed mutagenesis and expression of mutant and wildtype proteins in the yeast: Saccaromyces cerevisiae. Based on these data we suggest a mechanistic model for the (S)-cyanohydrin synthesis catalyzed by hydroxynitrile lyase from Hevea brasiliensis. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Griengl H |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kohlwein S D |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kratky C |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Schwab |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | IRRDB |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
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| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Physiology | 17/12/2021 | Volume 27, Issue 3 | Journals |