Heterografting induced DNA methylation polymorphisms in Hevea brasiliensis (Record no. 60285)

MARC details
000 -LEADER
fixed length control field 02265nam a2200229Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Thomas K.U
245 #0 - TITLE STATEMENT
Title Heterografting induced DNA methylation polymorphisms in Hevea brasiliensis
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher Planta
Year of publication 2018
300 ## - PHYSICAL DESCRIPTION
Number of Pages 579-589
520 ## - SUMMARY, ETC.
Summary, etc DNA methylation in response to stress may be associated with the alteration in gene transcription leading to morphological changes in plants. Rubber tree is commercially propagated by bud grafting where the scion of a high yielding variety is grafted on to a genetically divergent root stock. Still, significant levels of intraclonal variations exist among them. Epigenetic changes associated with heterografting may be partly responsible for this conundrum. In the present study, an attempt was made to identify the impact of divergent root stock on the epigenome of scion in grafted rubber plants. Heterografts were developed by grafting eye buds from a single polyembryony derived seedling on to genetically divergent root stocks of unknown parentage. The plants were uniformly maintained and their DNA was subjected to MSAP analysis. Polymorphic DNA methylation bands corresponding to CG as well as the plant-specific CHG types of methylation were observed. Cloning of selected polymorphic regions and bisulfite sequencing confirmed the presence of methylation in the promoter and coding region of important genes including an LRR receptor kinase gene. Since divergent root stock is the major factor differentiating the grafted plants, the changes in DNA methylation patterns might have been triggered by the interaction between the two genetically different tissues of stock and scion. The study assumes importance in Hevea, because accumulation and maintenance of epigenetic changes in functional genes and promoters during subsequent cycles of vegetative propagation may contribute towards intraclonal variability eventually leading to altered phenotypes.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term DNA methylation
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Epigenetic changes
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Hevea brasiliensis
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Intraclonal variability
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Stock-scion interaction
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Ravindran M
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Rekha K
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Saha T
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier Online article
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Journals
Holdings
Withdrawn status Lost status Damaged status Not for loan Home library Current library Shelving location Date acquired Serial Enumeration / chronology Koha item type
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