Genetic tansformation of Hevea brasiliensis with hman arial ntriuretic Factor (Record no. 59861)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01659nam a2200217Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Sunderasan E |
| 245 #0 - TITLE STATEMENT | |
| Title | Genetic tansformation of Hevea brasiliensis with hman arial ntriuretic Factor |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Journal of Rubber Research |
| Year of publication | 2012 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 255-264 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Agrobacterium mediated genetic transformation was performed on anther callus of Hevea cultivar GLI with a gene encoding human atrial natriuretic factor (HANF), a peptide hormone that is involved in regulating cardiac blood pressure. The gene constructs containing four different lengths of the indigenous hevein promoter, also incorporated the marker nptII for selection of transformants. Thus far 28 plants were regenerated and their bud-grafted generations were successfully transferred to the soil; all transcript in leaf samples was detected by RT-PCR in a number of original transformants and their vegetative generations, and the authenticity of the amplicon was confirmed by nucleotide sequencing. Polyclonal anti-atrial natriuretic peptide detected a band in the low molecular weigh region in western- immunoblot of leave protein of an original transformed plant, while MALDITOF-TOF and database searches of the corresponding protein band on SDS-gel revealed matches to atrial natriuretic peptide in the C-terminus of HANF. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | genetic transformation |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | human atrial natriuretic factor |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | protein mass-spectra analysis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | RT-PCR |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Arokiaraj P |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Azharuddin Azlina |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Badaruddin EBadrul |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Genome | 30/07/2013 | Volume 15, Issue 4 | Journals |