Expression of stress tolerance in transgenic callus integrated with osmotin gene in Hevea brasiliensis (Record no. 59178)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02002nam a2200265Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Rekha K |
| 245 #0 - TITLE STATEMENT | |
| Title | Expression of stress tolerance in transgenic callus integrated with osmotin gene in Hevea brasiliensis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Rubber Science |
| Year of publication | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 140-152 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Stressful environment is a limiting factor for rubber productivity in the traditional rubber growing areas and limits the expansion of rubber cultivation to newer areas in several rubber producing countries, including India. Conventional methods of Hevea breeding take several years to produce plants with desirable traits. Genetic transformation offers a viable approach for producing plants with desired traits within a short period of time. Since osmotin gene is reported to impart tolerance to abiotic and biotic stress, the present study on Agrobacterium mediated gene transfer was carried out with the gene encoding osmotin protein. Osmotin gene construct with CaMV 35S promoter and kanamycin as selectable marker was used for the purpose. Transformation frequency of 48 per cent was obtained from anther derived embryogenic calli. Molecular analysis with PCR and RT-PCR could confirm the integration of gene and its expression. The calli were subjected to PEG as well as salt stress for fixed intervals. Higher proline accumulation in tansgenic cell lines compared to control was observed under PEG stress. Transgenic cell lines could tolerate higher concentration (50 mM). The regenerated plants from these transgenic lines are expected to perform well under stressful environments. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Genetic transformation |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Osmotin |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Proline estimation |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Stress tolerance |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Akshara S R |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jayashree R |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mathew S A |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nazeem P A |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sobha S |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sushamakumari S |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Venkatachalam P |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 01/01/2021 | Volume 29, Issue 2 | Journals |