Expression analysis of rubber biosynthetic pathway genes in Hevea brasiliensis (Record no. 59169)

MARC details
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fixed length control field 02203nam a2200253Ia 4500
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Personal name Ambily P.K
245 #0 - TITLE STATEMENT
Title Expression analysis of rubber biosynthetic pathway genes in Hevea brasiliensis
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher Journal of Plantation Crops
Year of publication 2018
300 ## - PHYSICAL DESCRIPTION
Number of Pages 102-111
520 ## - SUMMARY, ETC.
Summary, etc Hevea brasiliensis, the primary commercial source of natural rubber (cis-1, 4-polyisoprene), is a fundamental raw material used for manufacturing a wide range of industrial and domestic rubber products in automobile, medical and defense industries. In Hevea, biosynthesis of rubber takes place through mevalonate pathway. Clonal variations in the productivity of rubber may be the result of variations in the activities of the enzymes involved in rubber biosynthesis in different Hevea clones. In this study, expression of 14 genes corresponding to enzymes/regulatory proteins involved in rubber biosynthesis was analyzed in high and low latex yielding clones of Hevea brasiliensis. The level of expression of HbSUT3, a sucrose transporter and enzymes related to the synthesis of rubber such as hydroxymethyl glutaryl-CoA synthase (hmgs), HMG-CoA reductase (hmgr) and mevalonate diphosphate decarboxylase (MVD) were found to be significantly higher in high rubber yielding clones compared to the low rubber yielding clones. The higher expression of these genes might result in an increased supply of IPP, the isoprenoid monomer, required for rubber biosynthesis. Expression of genes in the downstream rubber biosynthetic pathway such as FPPS, RuT and REF2 were also found to be significantly higher in high rubber yielding clones than low yielders. The results suggest that high rubber yield is associated with high expression of these genes and these genes can be used as markers for high yield potential in Hevea.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Gene expression
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Topical Term Hevea brasiliensis
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Topical Term HMG-CoA reductase
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Topical Term HMG-CoA synthase
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Topical Term Rubber biosynthesis
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Topical Term Sucrose transporter
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Annamalainathan K
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Personal name Jacob J
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Personal name Krishnakumar R
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Sreelatha S
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Thomas M
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Journals
Holdings
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