Evidence for association of a viroid with tapping panel dryness syndrome of rubber(Hevea brasiliensis) (Record no. 59042)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02265nam a2200217Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Ramachandran P |
| 245 #0 - TITLE STATEMENT | |
| Title | Evidence for association of a viroid with tapping panel dryness syndrome of rubber(Hevea brasiliensis) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Plant Disease |
| Year of publication | 2000 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 1155 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Tapping panel dryness (TPD) is one of the most destructive maladies affecting rubber plantations and is becoming a matter of serious concern. Reduced latex yield leading to total drying of the tapping panel is the obvious symptom. The cause of TPD syndrome is unknown but has been mostly attributed to abiotic causes. In India, the high yielding commercial clone RRII 105 is affected by TPD-affected trees show symptoms of bark scaling, cracking, drying, necrotic streaking, and browning of internal bark leading to the decay of internal tissues. Often prominant abnormal bulges on the lower part of tree trunks occur where the first panel begins to dry. Investigations on TPD-affected rubber samples did not reveal the association of fungus, bacterium, virus, or a protozoan. Total nucleic acid extracts purified from leaf and bark tissues of affected samples and analysed by polyacrylamide gel electrophoresis under denaturing conditions of low salt and high temperature showed the presence of nucleic acids similar in electrophoretic mobility to low molecular weight (LMW) RNA, of ~359 nucleotides such as potato spindle tuber viroid (PSTVd). The LMW nucleic acid detected from TPD-affected samples was found to be RNA based on its sensitivity to RNase and insensitivity to DNase, phenol, and heat treatments. The LMW RNA was purified and cloned in a pUC 19-derived vector by using primers specific to PSTVd (1). The cloned DNA, when random labeled and used as probe reacted specifically to nucleic acid extracts from TPD-affected rubber trees but not from healthy tissue in dot-blot hybridization assays. Based on the above findings, a viroid etiology for TPD syndrome is proposed. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Tapping panel dryness |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Francis L |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mathew J |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mathew N M |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mathur S |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sethuraj M R |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Varma A |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Physiology | 17/12/2021 | Volume 84, Issue 10 | Journals |