Efficient editing of an adenoviral vector genome with CRISPR/Cas9 (Record no. 58427)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01524nam a2200253Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Qiang Li |
| 245 #0 - TITLE STATEMENT | |
| Title | Efficient editing of an adenoviral vector genome with CRISPR/Cas9 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Indian Journal of Microbiology |
| Year of publication | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 91-95 |
| 500 ## - GENERAL NOTE | |
| General note | Source Year: 2021 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Immunotherapy based on genetic modification of T cells has played an important role in the treatment of tumors and viral infections. Moreover, adenoviral vectors engineered with improved safety due to their inability to engineered with improved safety due to their inability to integrate into the host genome have been key in the clinical application of T cells therapy. However, the commonly used adenoviral vector Ad5 exhibits low efficiency of infection of human T cells and the details of the intracellular trafficking pathway of adenoviral vectors in human primary T cells remains unclear. Resolution of these issues will depend on successful modification of the adenoviral vector. To this end, here we describe the successful establishment of a simple and efficient method for editing adenoviral vectors in vitro using the CRISPR-Cas9 gene editing system to target the adinoviral fiber gene. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Adenoviral vector |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | CRISPR-Cas9 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | T-cell therapy |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen Z |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen Z |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gong C-y |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shao Hong-w |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang H |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yang J-x |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang W-f |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 17/12/2021 | Volume 61, Issue 1 | Journals |