Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Mull Arg. plants (Record no. 58424)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01804nam a2200241Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Blanc Geraldine |
| 245 #0 - TITLE STATEMENT | |
| Title | Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Mull Arg. plants |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Plant Cell Reports |
| Year of publication | 2006 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 724-733 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | An efficient procedure for producing transgenic Hevea brasiliensis callus and plants lines from clone PB 260 was established with Agrobacterium tumefaciens using strain EHA 105 harbouring the vector pCAMBIA 2301. Transformation capacity and competence of the embryogenic calli were improved after two cycles of cryopreservation. When the cocultivation temperature was reduced from 27 to 20 oC, the duration of this phase could be increased uo to 7 days, promoting an increase in GUS activity. These transformation conditions led to the isolation of 24 callus lines resistant to paromomycin, which is used as a selection agent. Nineteen of these lines revealed the existence of one to four copies of T-DNA by Southern-blot analysis. Nine of them were transferred for regeneration by somatic embryogenesis. Three hundred seventy-four transgenic plants have thus been generated from six independent lines bearing 1,2 or 3 copies of T-DNA. The efficiency and reproducibility of this method means that functional characterization of genes involved in natural rubber production can be envisaged. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Agrobacterium tumefaciens |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Cocultivation |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Cryopreservation |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Genetic transformation |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | GUS |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Temperature |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Baptiste Christelle |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Martin Florence |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Montoro Pascal |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Oliver Gerald |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 17/12/2021 | Volume 24, Issue 12 | Journals |