Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Muell. Arg) (Record no. 57833)

MARC details
000 -LEADER
fixed length control field 01579nam a2200217Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Lardet Ludovic
245 #0 - TITLE STATEMENT
Title Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Muell. Arg)
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher Plant Cell Reports
Year of publication 2007
300 ## - PHYSICAL DESCRIPTION
Number of Pages 559-569
520 ## - SUMMARY, ETC.
Summary, etc A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The study showed that reducingthe CaCl2 concentration of the pre-culture medium from 9mM to 1 or 0 mM CaCl2 before cryopreservation promoted post-thaw callus growth, 1 mM being the optimum CaCl2 concentration for embryo regeneration. Post-thaw callus proliferation decreased in line with the increase of planted callus weight. The effect of cryopreservation was assessed on 39 independent lines showing that cryopreservation did not affect embryogenic and plant regeneration for a majority of lines. The decrease in CaCl2 concentration of the pre-culture medium and the endogenous calcium content of the calli. It also highlighted the implication of tissue calcium content in cryotolerance. Callus water status and the different ways by which calcium could prevent cryoinjury is also discussed.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Calcium
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Cryopreservation
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Embryogenic culture
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Pre-culture
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Carron Marc-Philippe
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dessailly Florence
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Martin Florence
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Montoro Pascal
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Journals
Holdings
Withdrawn status Lost status Damaged status Not for loan Home library Current library Shelving location Date acquired Serial Enumeration / chronology Koha item type
      Journals RRII Library RRII Library Biotechnology 05/06/2007 Volume 26, Issue 5 Journals