Effect of colchicine on callus induction from isolated microspores in Hevea brasiliensis (Record no. 57749)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 01430nam a2200217Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Jayasree R |
| 245 #0 - TITLE STATEMENT | |
| Title | Effect of colchicine on callus induction from isolated microspores in Hevea brasiliensis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Plant Cell Biotechnology and Molecular Biology |
| Year of publication | 2007 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 89-92 |
| 500 ## - GENERAL NOTE | |
| General note | Source Year: 2007 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | A method for the induction of haploid callus from isolated microspores of Hevea brasiliensis has been developed. The growth promoting effects of colchicine on callus formation was proved. Anthers at the late uni-nucleate to the early bi-nucleate stage of the microspores were pretreated at 4 degree C for 5-7 days before microspore isolation. Different durations of colchicine exposure (24-96 hrs) as well as varying concentrations (100-1000 mg/l) were tested after the pre-culture. The maximum response was noticed when the microspores were pre-cultures in 100 mg/l colchicine for 96 hrs. It was observed that a high sucrose concentration (180g/l) favoures callus growth in the presence of the growth regulators, kinetin and zeatin (3.0 mg/l). This is a major step towards utilization of haploid technology in Hevea breeding. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Androgenesis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Colchicine |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Isolated microspores |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jayasree P K |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rekha K |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sanju R |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Thulaseedharan A |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 29/10/2008 | Volume 8, Issue 02-Jan | Journals |