Development, characterization, and cross-species/genera transferability of SSR markers for rubber tree (Hevea brasiliensis) (Record no. 57086)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02289nam a2200241Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Yu Fei |
| 245 #0 - TITLE STATEMENT | |
| Title | Development, characterization, and cross-species/genera transferability of SSR markers for rubber tree (Hevea brasiliensis) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Plant Cell Reports |
| Year of publication | 2011 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 335-344 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Genomic simple sequence repeat (SSR) markers are particularly valuable in studies of genetic diversity, evolution, genetic linkage map construction, quantitative trait loci tagging, and marker-assisted selection because of their multi-allelic nature, reproducibility, co-dominant inheretance, high abundance, and extensive genome coverage. The traditional methods of SSR marker development, such as genomic-SSR hybrid screening and microsatellite enrichment, have the disadvantages of high cost and complex operation. The selectively amplified microsatellite method is less costly and highly efficient as well as being simple and convenient. In this study, 252 sequences with SSRs were clones from the rubber tree (Hevea brasiliensis) genome from which 258 SSR loci were obtained. The average repeat number was six. There were only 10 (3.9;) mononucleotide, trinucleotide, and pentanucleotide repeats, whereas the remaining 248 (96.1;) were dinucleotide repeats, including 128 (49.6;) GT/CA repeats, 118 (45.7;) GA/CT repeats, and 2 (0.8;) AT/TA repeats. A total of 126 primer pairs (see ESM) were successfully designed of which 36 primer pairs generated polymorphic products from 12 accessions of the cultivated species, 4 related species, and 3 species of the family Euphorbiaceae. In addition, investigations based on four genomic SSRs (GAR4, ACR22, CTR25, and GTR28) by cloning and sequencing provided evidence for cross-species/genera applicability, and homologous sequences were obtained from the rubber tree and Euphorbiaceae. Further analysis about the variation of the flanking regions of the four markers was carried out. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Development |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Flanking regions |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Genomic SSR |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Polymorphism |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Variation |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Feng Su-Ping |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li Wei-Guo |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang Bao-Hua |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang Jing-Yi |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wu Yao-Ting |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 05/08/2011 | Volume 30, Issue 3 | Journals |