Cryopreservation of embryogenic calluses of two commercial clones of Hevea brasiliensis (Record no. 56587)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01369nam a2200217Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Engelmann F |
| 245 #0 - TITLE STATEMENT | |
| Title | Cryopreservation of embryogenic calluses of two commercial clones of Hevea brasiliensis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Cryo-letters |
| Year of publication | 1997 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 107-116 |
| 500 ## - GENERAL NOTE | |
| General note | Source Year: 1998 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Two cryopreservation protocols, one using a classical freezing process and the other a simplified freezing process, were developed for embryogenic calluses of a commercial clone of Hevea, PR107. After preculture with 1 M sucrose and 10;dimethyl sulfoxide, embryogenic calluses were frozen in a programmable freezer at 0.2 degree C min-1 down to -40 degree C, or in a simple device consisting of an isopropanol bath enclosed in a polystyrene box, placed in a -80 degree C deep-freezer, thus achieving an average cooling rate of 0.2 degree C min-1 down to -40 degree C. High survival and rapid regrowth, as well as production of somatic embryos, were obtained with calluses cryopreserved using both freezing protocols. The simple freezing protocols was successfully used with a second commercial clone. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Cryopreservation |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Carron M P |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chabrillange N |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Etienne H |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lartaud M |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | IRRDB |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 17/12/2021 | Volume 18, Issue 2 | Journals |