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Cloning and characterization of B-1, 3-glucanase gene from different clones of Hevea brasiliensis (Record no. 55770)

MARC details
000 -LEADER
fixed length control field	02274nam a2200181Ia 4500
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name	Supriya R
245 #0 - TITLE STATEMENT
Title	Cloning and characterization of B-1, 3-glucanase gene from different clones of Hevea brasiliensis
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher	Placrosym XVII, 2006, Kochi, India. Journal of Plantation Crops, 34 (3). Indian Cardamom Research Institute (Spices Board), Idukki, India, pp.572-575.
520 ## - SUMMARY, ETC.
Summary, etc	The relative tolerance of plants towards a particular disease/pathogen is mainly determined by the interactions between the disease resistant R genes and the associated pathogen avirulence avr genes, affecting the plant. B-1, 3-glucanase enzyme, which belongs to the category of PR-2 (pathogenesis related proteins) family is known to be involved in the plant defence reactions against pathogens like Phytophthora causing abnormal leaf fall disease (ALF) in rubber plantations. The present work was undertaken to characterize the genomic DNA sequences coding for B-1, 3-glucanase from different clones of Hevea brasiliensis. High quality genomic DNA was isolated from Hevea clones, FX 516, RRII 105, RRII 33, PR 107, PB 86 and RRIM 701. B-1, 3-glucanase genomic sequences were PCR amplified using oligonucleotide primers designed based on previously reported sequences. A PCR product with approximately 1.3 Kb in size was amplified from all the clones. The isolated gene was later cloned in the Topo TA* cloning vector, sequenced and analysed for sequence variations. The result shows major variations within the intronic sequences. CT repeats within the intronic sequences show deletion/insertions among the clones studied. Although, the differences occur in the number of deletions, the location is almost similar. Only minor variations in nucleotides were observed in the exons. Though, the exact role of introns in a particular gene is not completely known, it is reported that through alternative splicing and non-sense mediated mRNA decay; the introns play an important role in the expression of a particular gene.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Abnormal leaf fall disease
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	B-1, 3-glucanase
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Genomic sequences
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term	Intron deletions
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Saleena A
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Thulaseedharan A
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type	Journals

Holdings
Withdrawn status	Lost status	Damaged status	Not for loan	Home library	Current library	Shelving location	Date acquired	Koha item type
			Journals	RRII Library	RRII Library	Biotechnology	16/01/2007	Journals