Cloning and characterization of a novel cysteine protease gene (HbCP1) from Hevea brasiliensis (Record no. 55769)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01495nam a2200205Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Peng Shi-Qing |
| 245 #0 - TITLE STATEMENT | |
| Title | Cloning and characterization of a novel cysteine protease gene (HbCP1) from Hevea brasiliensis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Journal of Bioscience |
| Year of publication | 2008 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 681-690 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | The full-length cDNA encoding a cysteine protease, designated HbCP1, was isolated for the first time from Hevea brasiliensis by the rapid amplification of cDNA ends (RACE) method HbCP1 contained a 1371 bp open reading frame encoding 457 amino acids. The deduced HbCP1 protein, which showed high identity to cysteine proteases of other plant species, was predicted to pocesses a putative repeat in toxin (RTX) domain at the N-terminal and a granulin (GRAN) domain at the C-terminal. Southern blot analysis indicated that the HbCP1 gene is present as a single copy in the rubber tree. Transcription pattern analysis revealed that HbCP1 had high transcription in laticifer, and low transcription in bark and leaf. The transcription of HbCP1 in latex was induced by ethylene and tapping. Cloning of the HbCP1 gene will enable us to further understand the molecular characterization of cycteine protease and its possible function in the rubber tree. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Cysteine protease |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Gene expression |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Latex |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Stress |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li Hui-Liang |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tian Wei-Min |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhu Jia-Hong |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 23/01/2009 | Volume 33, Issue 5 | Journals |